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rdf:type |
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lifeskim:mentions |
umls-concept:C0014597,
umls-concept:C0017262,
umls-concept:C0028135,
umls-concept:C0033809,
umls-concept:C0035820,
umls-concept:C0040648,
umls-concept:C0079633,
umls-concept:C0205224,
umls-concept:C0521346,
umls-concept:C0521447,
umls-concept:C0851285
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pubmed:issue |
8
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pubmed:dateCreated |
1999-8-12
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pubmed:abstractText |
Persistent infection with Pseudomonas aeruginosa increases interleukin-8 (IL-8) levels and causes dense neutrophil infiltrations in the airways of patients with chronic airway diseases. Recently, we have reported that nitrite reductase from P. aeruginosa induces the production of IL-8 in respiratory cells, including bronchial epithelial cells. To determine the molecular mechanism(s) of nitrite reductase-induced IL-8 expression in respiratory cells, A549 epithelial cells were transfected with plasmids containing serial deletions of the 5'-flanking region of the IL-8 gene and then exposed to nitrite reductase. Nitrite reductase significantly enhanced IL-8 gene promoter-driven reporter activity. This increased IL-8 gene expression was inhibited by mutating the nuclear factor-kappaB (NF-kappaB) binding element. Nitrite reductase enhanced nuclear localization of the NF-kappaB binding complex. Furthermore, nitrite reductase induced the degradation of IkappaBalpha, the major cytoplasmic inhibitor of NF-kappaB, and the expression of IkappaBalpha mRNA. These data support the critical role of the activation of NF-kappaB in nitrite reductase-induced IL-8 gene expression in airway epithelium.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-10103183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1331059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1357002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-14002367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-14002372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-175022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1752942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1801651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1856209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1870988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1918068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-1931078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-21765000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-2250017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-2500307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-2506077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-3140380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-3298433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-4198683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7510691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7525815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7580291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7593606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7627968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7888182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7927669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-7946394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8011280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8035808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8207232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8234276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8262046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8282796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8413215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-8420410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-9188651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-9199432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-9271387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10417151-9593012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3872-8
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pubmed:dateRevised |
2011-8-31
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pubmed:meshHeading |
pubmed-meshheading:10417151-DNA-Binding Proteins,
pubmed-meshheading:10417151-Epithelial Cells,
pubmed-meshheading:10417151-Gene Expression Regulation,
pubmed-meshheading:10417151-Humans,
pubmed-meshheading:10417151-I-kappa B Proteins,
pubmed-meshheading:10417151-Interleukin-8,
pubmed-meshheading:10417151-Lung,
pubmed-meshheading:10417151-NF-kappa B,
pubmed-meshheading:10417151-Nitrite Reductases,
pubmed-meshheading:10417151-Promoter Regions, Genetic,
pubmed-meshheading:10417151-Pseudomonas aeruginosa,
pubmed-meshheading:10417151-Transcription Factor AP-1,
pubmed-meshheading:10417151-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Essential role of transcription factor nuclear factor-kappaB in regulation of interleukin-8 gene expression by nitrite reductase from Pseudomonas aeruginosa in respiratory epithelial cells.
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pubmed:affiliation |
Department of Preventive Medicine and AIDS Research, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. n-mori@net.nagasaki-u.ac.jp
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pubmed:publicationType |
Journal Article,
Retracted Publication,
Research Support, Non-U.S. Gov't
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