| pubmed-article:10401703 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C0020205 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C0039748 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:10401703 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:10401703 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10401703 | pubmed:dateCreated | 1999-9-2 | lld:pubmed |
| pubmed-article:10401703 | pubmed:abstractText | Previous experiments have demonstrated differences in various follicular characteristics between the prolific Chinese Meishan (MS) pig and European Large-White (LW) hybrids and the present experiment was designed to compare the cAMP response to LH by granulosa and theca cells in vitro between the two breeds. Ovaries were recovered from MS (n = 7) and LW hybrid (n = 8) gilts on the day before predicted oestrus and the 12 largest follicles dissected. Quadruplicate aliquots of granulosa cells or minced theca tissue were incubated for 1 h in the presence of 0, 0.1, 1.0, 10, 100 or 1000 ng/ml LH and cAMP production measured. Follicles from MS gilts were smaller (5.9 vs. 7.7 mm; P < 0.001), contained less fluid (81.5 vs. 177.4 microl; P < 0.001), had fewer granulosa cells (3.8 vs. 5.3 x 10(6); P < 0.01) and less theca tissue (30.3 vs. 50.5 mg; P < 0.05) than those from LW hybrid animals. Mean follicular fluid oestradiol concentration was > or = 149 ng/ml in all animals and tended to be higher in the MS follicles (P = 0.07). LH stimulated cAMP production by granulosa and theca cells from both breeds (P < 0.001). Although there was no overall breed effect for the granulosa cells, there was a significant (P < 0.001) interaction between LH dose and breed in the granulosa cells, whether cAMP production was expressed per 10(6) cells or per follicle. In the theca incubations, cAMP production by MS tissue was higher (P < 0.01) when results were expressed per mg tissue and again there was an interaction (P < 0.001) between LH dose and breed whether cAMP production was expressed per mg tissue or per follicle. For both tissue types, MS follicles produced more cAMP at the higher LH doses. In conclusion, this study has shown that MS granulosa and theca tissue respond differently to increasing doses of LH in terms of cAMP production in vitro compared to LW hybrid tissue and this supports previous suggestions of enhanced maturity of MS follicles in the late follicular phase. | lld:pubmed |
| pubmed-article:10401703 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10401703 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10401703 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10401703 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10401703 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10401703 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10401703 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10401703 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10401703 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10401703 | pubmed:month | May | lld:pubmed |
| pubmed-article:10401703 | pubmed:issn | 0378-4320 | lld:pubmed |
| pubmed-article:10401703 | pubmed:author | pubmed-author:HunterM GMG | lld:pubmed |
| pubmed-article:10401703 | pubmed:author | pubmed-author:PictonH MHM | lld:pubmed |
| pubmed-article:10401703 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10401703 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:10401703 | pubmed:volume | 56 | lld:pubmed |
| pubmed-article:10401703 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10401703 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10401703 | pubmed:pagination | 67-78 | lld:pubmed |
| pubmed-article:10401703 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:meshHeading | pubmed-meshheading:10401703... | lld:pubmed |
| pubmed-article:10401703 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10401703 | pubmed:articleTitle | Comparison of cyclic AMP production in response to LH by granulosa and theca cells from Meishan and Large-White hybrid gilts. | lld:pubmed |
| pubmed-article:10401703 | pubmed:affiliation | Division of Animal Physiology, School of Biological Sciences, University of Nottingham, Loughborough, UK. morag.hunter@nottingham.ac.uk | lld:pubmed |
| pubmed-article:10401703 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10401703 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10401703 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
