Linked Life Data

10362069

Source:http://linkedlifedata.com/resource/pubmed/id/10362069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-7-9
pubmed:abstractText
We previously reported the preparation and characterization of an antibody against membrane fraction of autolysosomes from rat liver (J. Histochem. Cytochem. 38, 1571-1581, 1990). Immunoblot analyses of total membrane fraction of a rat hepatoma cell line, H-4-II-E cells by this antibody suggested that H-4-II-E cells expressed several autolysosomal proteins, including a protein with apparent molecular weight of 60 kDa. It was suggested that this 60 kDa protein was a peripheral membrane protein, because it was eluted from the membrane by sodium carbonate treatment. We prepared an antibody against this 60 kDa protein by affinity purification method, and examined its behavior during induction of autophagy. Autophagy was induced by transferring the cells from Dulbecco's modified Eagle medium (DMEM) containing 12% fetal calf serum into Hanks' balance salt solution. In DMEM, the 60 kDa protein showed diffused immunofluorescence pattern, and immunoelectron microscopy suggested that this protein was located on the extracellular side of the plasma membrane. After inducing autophagy, the immunofluorescence configuration of the 60 kDa protein changed from the diffused pattern to a granulous one. Immunoelectron microscopy showed that the 60 kDa protein was localized on the luminal side of the limiting membrane of autolysosomes and endosomes. In the presence of bafilomycin A1 which prevents fusion between autophagosomes and lysosomes, the 60 kDa protein was localized on the limiting membrane of the autophagosomes and endosomes. These results suggest that the 60 kDa protein is transported from the plasma membrane to the autophagosome membrane through the endosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0386-7196
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10362069-Animals, pubmed-meshheading:10362069-Anti-Bacterial Agents, pubmed-meshheading:10362069-Antibodies, pubmed-meshheading:10362069-Autophagy, pubmed-meshheading:10362069-Biological Markers, pubmed-meshheading:10362069-Blotting, Western, pubmed-meshheading:10362069-Carbonates, pubmed-meshheading:10362069-Cell Membrane, pubmed-meshheading:10362069-Culture Media, pubmed-meshheading:10362069-Endosomes, pubmed-meshheading:10362069-Fluorescent Antibody Technique, pubmed-meshheading:10362069-Intracellular Membranes, pubmed-meshheading:10362069-Isoelectric Point, pubmed-meshheading:10362069-Liver, pubmed-meshheading:10362069-Liver Neoplasms, Experimental, pubmed-meshheading:10362069-Lysosomes, pubmed-meshheading:10362069-Macrolides, pubmed-meshheading:10362069-Membrane Proteins, pubmed-meshheading:10362069-Microscopy, Immunoelectron, pubmed-meshheading:10362069-Molecular Weight, pubmed-meshheading:10362069-Phagosomes, pubmed-meshheading:10362069-Rats, pubmed-meshheading:10362069-Solubility, pubmed-meshheading:10362069-Tumor Cells, Cultured
pubmed:year
1999
pubmed:articleTitle
A 60 kDa plasma membrane protein changes its localization to autophagosome and autolysosome membranes during induction of autophagy in rat hepatoma cell line, H-4-II-E cells.
pubmed:affiliation
Department of Physiology, Kansai Medical University, Moriguchi, Osaka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't