Linked Life Data

10318758

Source:http://linkedlifedata.com/resource/pubmed/id/10318758

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-9-9
pubmed:abstractText
Vesicle transport requires the recruitment of cytosolic proteins to specific membrane compartments. We have previously characterised a brefeldin A-sensitive trans-Golgi network-localised protein (p230) that is associated with a population of non-clathrin-coated vesicles. p230 recycles between the cytosol and the cytoplasmic face of buds/vesicles of trans-Golgi network membranes in a G protein-regulated manner. Identifying the mechanism responsible for Golgi targeting of p230 is important for the elucidation of its function. By transfection of COS cells with deletion mutants of p230 we here demonstrate that the C-terminal domain is necessary for targeting to the Golgi. Furthermore, the C-terminal 98 amino acid domain of p230 attached to the green fluorescent protein (GFP-p230-C98aa) was efficiently Golgi-localised in transfected COS cells. Deletion mutants of GFP-p230-C98aa together with alanine scanning mutagenesis identified a minimum stretch of 42 amino acids that is essential for Golgi targeting, suggesting that the conformation of the domain is critical for efficient targeting. In COS cells expressing high levels of GFP-p230-C98aa fusion protein, endogenous p230 was no longer associated with Golgi membranes, suggesting that the GFP fusion protein and endogenous p230 may compete for the same membrane target structures. The Golgi binding of GFP-p230-C98aa is brefeldin A-sensitive and is regulated by G proteins. These studies have identified a minimal sequence responsible for specific targeting of p230 to the Golgi apparatus, which displays similar membrane binding characteristics to wild-type p230.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
112 ( Pt 11)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1645-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10318758-Alternative Splicing, pubmed-meshheading:10318758-Amino Acid Sequence, pubmed-meshheading:10318758-Animals, pubmed-meshheading:10318758-Autoantigens, pubmed-meshheading:10318758-Binding Sites, pubmed-meshheading:10318758-Brefeldin A, pubmed-meshheading:10318758-COS Cells, pubmed-meshheading:10318758-Golgi Apparatus, pubmed-meshheading:10318758-Green Fluorescent Proteins, pubmed-meshheading:10318758-Guanosine 5'-O-(3-Thiotriphosphate), pubmed-meshheading:10318758-HeLa Cells, pubmed-meshheading:10318758-Humans, pubmed-meshheading:10318758-Luminescent Proteins, pubmed-meshheading:10318758-Membrane Proteins, pubmed-meshheading:10318758-Molecular Sequence Data, pubmed-meshheading:10318758-Protein Synthesis Inhibitors, pubmed-meshheading:10318758-Recombinant Fusion Proteins, pubmed-meshheading:10318758-Structure-Activity Relationship
pubmed:year
1999
pubmed:articleTitle
The Golgi-targeting sequence of the peripheral membrane protein p230.
pubmed:affiliation
Department of Pathology and Immunology, Monash University Medical School, Melbourne, Victoria, Australia 3181.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't