Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1977-2-16
|
| pubmed:abstractText |
The influenza A/Hong Kong/68-ts-1[E] virus (shutoff temperature, 38 C), which possesses many characteristics desirable in a vaccine virus, was used as a donor of its two temperature-sensitive (ts) lesions to the antigenically divergent influenza A/Udorn/72 wild-type virus. Two subsets of Udorn/72-ts-1[E] recombinant viruses were evaluated in seronegative volunteers (serum titer of hemagglutination-inhibiting antibody, less than or equal 1:8). The first subset, represented by clone 13, possessed a shutoff temperature of 39 C and only one of the two ts lesions; this virus was insufficiently attenuated for use in humans. The other subset, represented by clones 16 and 24, possessed both ts lesions and a shutoff temperature of 38 C, like that of its Hong Kong/68-ts-1[E] parent. This subset, also like its ts-1[E] parent, was adequately attenuated, nontransmissible, and protective against intranasal challenge with wild-type Udorn/72 virus. The attenuation manifested by the ts mutants was not a result of their cloning in bovine kidney tissue or replication in eggs. The results suggest that the Hong Kong/68-ts-1[E] virus can be considered for use as a master strain for donation of ts lesions and thus could bring about predictable attenuation of new wild-type influenza A viruses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1899
|
| pubmed:author |
pubmed-author:BettsR FRF,
pubmed-author:BlacklowN RNR,
pubmed-author:CaplanE SES,
pubmed-author:ChanockR MRM,
pubmed-author:DouglasR GRG,
pubmed-author:GwaltneyJ MJMJr,
pubmed-author:LevineM MMM,
pubmed-author:MurphyB RBR,
pubmed-author:ParrinoT ATA,
pubmed-author:RichmanD DDD,
pubmed-author:RossF TFT
|
| pubmed:issnType |
Print
|
| pubmed:volume |
134
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
585-94
|
| pubmed:dateRevised |
2005-11-22
|
| pubmed:meshHeading |
pubmed-meshheading:1003014-Antibodies, Viral,
pubmed-meshheading:1003014-Hemagglutination Inhibition Tests,
pubmed-meshheading:1003014-Humans,
pubmed-meshheading:1003014-Influenza, Human,
pubmed-meshheading:1003014-Influenza A virus,
pubmed-meshheading:1003014-Mutation,
pubmed-meshheading:1003014-Neuraminidase,
pubmed-meshheading:1003014-Recombination, Genetic,
pubmed-meshheading:1003014-Temperature
|
| pubmed:year |
1976
|
| pubmed:articleTitle |
Temperature-sensitive mutants of influenza A virus. XII. Safety, antigenicity, transmissibility, and efficacy of influenza A/Udorn/72-ts-1[E] recombinant viruses in human adults.
|
| pubmed:publicationType |
Journal Article
|