Linked Life Data

10027649

Source:http://linkedlifedata.com/resource/pubmed/id/10027649

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-8-2
pubmed:abstractText
Interferons (IFNs) stimulate gene expression to mediate their biological actions. A multimeric transcription factor consisting of STAT1, STAT2 and p48, a DNA binding protein, regulates IFN-alpha/beta stimulated gene expression. Since the cellular level of p48 is also increased by pre-treatment of cells with IFN-gamma, it is also known as ISGF3gamma. To understand how IFN-gamma regulates the expression of the p48 gene, we have previously isolated and characterized the promoter of murine p48 gene and identified a novel gamma-IFN activated transcriptional element (GATE). In this study using several mutant constructs of p48 promoter we have determined that the same element responds to IFN-alpha/beta treatment. Relatively high doses of IFN-alpha/beta compared to IFN-gamma are required for the induction of p48 promoter. This ability of IFN-alpha/beta to regulate GATE dependent gene expression is linked to the activation of a factor induced by IFN-alpha. However, IFN-gamma induces the binding of two gamma-IFN inducible factors (GIFs) to GATE. The IFN-alpha inducible GATE binding factor is not recognized by specific antibodies raised against the known IFN-regulated factors. It is likely IFN-gamma is a stronger inducer of this gene because it activates two GIFs. GATE-like elements present in hither to undefined IFN-stimulated genes may control IFN-responses in a unique manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0166-3542
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10027649-Animals, pubmed-meshheading:10027649-Blotting, Northern, pubmed-meshheading:10027649-Cells, Cultured, pubmed-meshheading:10027649-DNA-Binding Proteins, pubmed-meshheading:10027649-Gene Expression Regulation, pubmed-meshheading:10027649-Interferon-Stimulated Gene Factor 3, pubmed-meshheading:10027649-Interferon-Stimulated Gene Factor 3, gamma Subunit, pubmed-meshheading:10027649-Interferon-alpha, pubmed-meshheading:10027649-Interferon-beta, pubmed-meshheading:10027649-Interferons, pubmed-meshheading:10027649-Macrophages, pubmed-meshheading:10027649-Mice, pubmed-meshheading:10027649-Promoter Regions, Genetic, pubmed-meshheading:10027649-Rabbits, pubmed-meshheading:10027649-Response Elements, pubmed-meshheading:10027649-Transcription Factors, pubmed-meshheading:10027649-Transcriptional Activation
pubmed:year
1999
pubmed:articleTitle
Regulation of interferon-alpha/beta-stimulated gene expression through the gamma-activated transcriptional element.
pubmed:affiliation
Greenebaum Cancer Center, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.