Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-3-12
pubmed:abstractText
The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to B. pertussis (infected as adults, occupational exposure, immunized with an acellular vaccine, or no identified exposure) against a wild-type strain and a BrkA complement-sensitive mutant were evaluated. All of the sera killed the BrkA mutant, suggesting past exposure to B. pertussis or cross-reactive organisms. Several samples had no or minimal activity against the wild type. All of the sera collected from the infected and occupationally exposed individuals but not all of the sera from vaccinated individuals had bactericidal activity against the wild-type strain, suggesting that some types of exposure can induce an immune response that can overcome the BrkA resistance mechanism. Adsorbing serum with the wild-type strain removed the bactericidal antibodies; however, adsorbing the serum with a lipopolysaccharide (LPS) mutant or an avirulent (bvg mutant) strain did not always result in loss of bactericidal activity, suggesting that antibodies to either LPS or bvg-regulated proteins could be bactericidal. All the samples, including those that lacked bactericidal activity, contained antibodies that recognized the LPS of B. pertussis. Bactericidal activity correlated best with the presence of the immunoglobulin G3 (IgG3) antibodies to LPS, the IgG subtype that is most effective at fixing complement.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-1377181, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-1452367, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-1596364, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-1642903, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-1699109, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-233166, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-2569447, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-2572561, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-3968450, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-7509316, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-7543907, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-7659478, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-7729920, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-7927748, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8050099, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8118532, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8153871, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8256198, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8300232, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8335349, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8423070, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8478113, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8557325, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8600536, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8811042, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8821935, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8849226, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-8894399, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9109161, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9129097, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9154547, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9169747, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9245820, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9284132, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9357448, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9453625, http://linkedlifedata.com/resource/pubmed/commentcorrection/10024590-9631546
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1424-31
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Characterization of human bactericidal antibodies to Bordetella pertussis.
pubmed:affiliation
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267-0524, USA. Alison.Weiss@uc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't