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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-3-25
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pubmed:databankReference |
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pubmed:abstractText |
Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-1310793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-1657641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-1739976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-1915298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-1924319,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-2055484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-2167835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-2253890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-2506439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-3012280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-3014435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-3097516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-3315231,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-3918018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-3924740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-6264467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-6366512,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-7498787,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-8529272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-8756637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-8923734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-8978031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-9111019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-9285828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10022891-9618445
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2044-50
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10022891-Amino Acid Sequence,
pubmed-meshheading:10022891-Base Sequence,
pubmed-meshheading:10022891-Culture Media,
pubmed-meshheading:10022891-DNA, Fungal,
pubmed-meshheading:10022891-DNA-Binding Proteins,
pubmed-meshheading:10022891-Fungal Proteins,
pubmed-meshheading:10022891-Gene Expression Regulation, Fungal,
pubmed-meshheading:10022891-Glucose,
pubmed-meshheading:10022891-Molecular Sequence Data,
pubmed-meshheading:10022891-Promoter Regions, Genetic,
pubmed-meshheading:10022891-Repressor Proteins,
pubmed-meshheading:10022891-STAT1 Transcription Factor,
pubmed-meshheading:10022891-Saccharomyces cerevisiae,
pubmed-meshheading:10022891-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:10022891-Trans-Activators,
pubmed-meshheading:10022891-Zinc Fingers
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pubmed:year |
1999
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pubmed:articleTitle |
Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Microbiology, College of Natural Sciences, Seoul National University, Seoul 151-742, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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